Treatment of brain cells called astrocytes, with the Indian curry spice, curcumin (turmeric) has been found to increase expression of the glutathione S-transferase and protect neurons exposed to oxidant stress.
Glutathione in Health and Disease
It is believed that glutathione has an important role to play in the prevention and treatment of disease.
It may in the future be considered as important to health as an alkaline diet, exercise and alkaline lifestyle.
Clinical tests show that raised glutathione levels may address some of the eight major health issues of our time. '
Click here for more about Glutathione and its benefits to our body.
Showing posts with label oxidative-stress. Show all posts
Showing posts with label oxidative-stress. Show all posts
Wednesday, January 23, 2008
Tuesday, January 22, 2008
Involvement of uric acid and glutathione antioxidant defenses
Insulin neuroprotection against oxidative stress in cortical neurons-Involvement of uric acid and glutathione antioxidant defenses.
In this study we investigated the effect of insulin on neuronal viability and antioxidant defense mechanisms upon ascorbate/Fe(2+)-induced oxidative stress, using cultured cortical neurons.
Insulin (0.1 and 10 muM) prevented the decrease in neuronal viability mediated by oxidative stress, decreasing both necrotic and apoptotic cell death.
Moreover, insulin inhibited ascorbate/Fe(2+)-mediated lipid and protein oxidation, thus decreasing neuronal oxidative stress.
Increased 4-hydroxynonenal (4-HNE) adducts on GLUT3 glucose transporters upon exposure to ascorbate/Fe(2+) were also prevented by insulin, suggesting that this peptide can interfere with glucose metabolism.
We further analyzed the influence of insulin on antioxidant defense mechanisms in the cortical neurons. Oxidative stress-induced decreases in intracellular uric acid and GSH/GSSG levels were largely prevented upon treatment with insulin.
Inhibition of phosphatidylinositol-3-kinase (PI-3K) or mitogen-induced extracellular kinase (MEK) reversed the effect of insulin on uric acid and GSH/GSSG , suggesting the activation of insulin-mediated signaling pathways.
Moreover, insulin stimulated glutathione reductase (GRed) and inhibited glutathione peroxidase (GPx) activities under oxidative stress conditions, further supporting that insulin neuroprotection was related to the modulation of the glutathione redox cycle.
Thus, insulin may be useful in preventing oxidative stress-mediated injury that occurs in several neurodegenerative disorders.
by: AI Duarte, MS Santos, CR Oliveira, AC Rego
Click here for more about Glutathione and its benefits to our body.
In this study we investigated the effect of insulin on neuronal viability and antioxidant defense mechanisms upon ascorbate/Fe(2+)-induced oxidative stress, using cultured cortical neurons.
Insulin (0.1 and 10 muM) prevented the decrease in neuronal viability mediated by oxidative stress, decreasing both necrotic and apoptotic cell death.
Moreover, insulin inhibited ascorbate/Fe(2+)-mediated lipid and protein oxidation, thus decreasing neuronal oxidative stress.
Increased 4-hydroxynonenal (4-HNE) adducts on GLUT3 glucose transporters upon exposure to ascorbate/Fe(2+) were also prevented by insulin, suggesting that this peptide can interfere with glucose metabolism.
We further analyzed the influence of insulin on antioxidant defense mechanisms in the cortical neurons. Oxidative stress-induced decreases in intracellular uric acid and GSH/GSSG levels were largely prevented upon treatment with insulin.
Inhibition of phosphatidylinositol-3-kinase (PI-3K) or mitogen-induced extracellular kinase (MEK) reversed the effect of insulin on uric acid and GSH/GSSG , suggesting the activation of insulin-mediated signaling pathways.
Moreover, insulin stimulated glutathione reductase (GRed) and inhibited glutathione peroxidase (GPx) activities under oxidative stress conditions, further supporting that insulin neuroprotection was related to the modulation of the glutathione redox cycle.
Thus, insulin may be useful in preventing oxidative stress-mediated injury that occurs in several neurodegenerative disorders.
by: AI Duarte, MS Santos, CR Oliveira, AC Rego
Click here for more about Glutathione and its benefits to our body.
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